We have dedicated space in the Earth and Environment Department at Boston University for our laboratory. This space was renovated in 2012, 2016, and 2019 to include dedicated locations for biogeochemical and ecological laboratory work as well as environmental molecular work. Additionally we have separate laboratory space dedicated solely to the development and bench testing of instruments such as the in situ porewater sampling system/underwater mass spectrometer and ultra portable gas sensors. We also have a wet lab which includes an environmental chamber with temperature and humidity control.

Fulweiler Lab Molecular Equipment: AirClean Systems AC600 Series Combination PCR Workstation  with vertical laminar flow air (pre-filter and HEPA filter) and a timed UV light for sterilization; BioRad T100™ Thermal Cycler  system which includes 96-well thermal cycler; BioRad Gel DOC EZ System with Image Lab™ software and stain specific sample trays (e.g., a try for GelGreen or any SYBR stain, a tray for Coomassie Blue, copper, silver, or zinc stains, and a tray for ultraviolet illumination); Promega Quantiflor Handheld fluorometer with two channels (UV and Blue) and a PCR tube adapter. Other equipment includes: Isotemp dry bath incubator, Isotemp water bath, standard submarine gel unit, FisherBiotech electrophoresis power supply, heavy duty vortex mixers, benchtop centrifuge, icemaker, and a Thermo Scientific -80 Freezer, Thermo Scientific Bio-Cane Cryogenic Vessel, Geneious Software package.

Ecological/Biogeochemical Equipment: membrane inlet mass spectrometer system (MIMS) with specially designed inlet system attached to a Balzers quadrupole MS; one in situ porewater sampling system/underwater mass spectrometer; a Shimadzu GC-2014 (with high sensitivity detectors (thermal conductivity: CO2, flame ionization detector: CH4, and electron capture: N2O); δ13C carbon isotope instruments for CO2 and CH4 concentration and isotope composition;  Picarro Gas Scouter G4301 for CO2 and CH4 concentrations, (quote requested; ordering fall 2022); a SEAL –Nutrient Autoanalyzer. We have a Unisense microprofiler which allows fine scale measurements of various dissolved gases, pH, etc. We have basic biogeochemical equipment including: Turner fluorometer, Hach dissolved oxygen, salinity, and pH probes, pH meters, redox meters, centrifuges, analytical balances, muffle furnaces, refrigerators, water baths, freezers, and ovens. Both water and soil/sediment samples can be analyzed in the laboratory. Nutrient analysis includes dissolved (NH4+, NO3- NO2-, Si, DON, DOP) and particulate forms. Gas analyses include N2, Ar, O2, CH4, N2O, CO2, and 28,29,30N2.  

BU Biology Core Facility: NanoQuant Multimode Microplate Reader, 7900 HT Fast Real-Time PCR by Applied Biosystems, StepOneTM Plus 96-well Real-Time PCR System by Applied Biosystems, Nanodrop UV Visible spectrophotometer, Primer Express Software v3.0 for building primers and probes using TaqManR and SYBR Green dye chemistries for gene quantification in RT-PCR applications. Additionally, these instruments come with technical support from BU and SOPS if needed.  

Boston University also has a core stable isotope laboratory that can carry out analyses on a variety of samples for stable isotopes as well as %C and %N. A discounted price list is available to in house customers. This facility includes a Finnigan Delta-S and two GV Instruments IsoPrime ratio mass spectrometers as well as elemental analyzers and associated peripherals.